Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
ACROBiosystems E. coli resDNA Quantitative Kit (qPCR)
Residual host cell DNA refers to fragments of DNA from the host organism that are left behind after a biological process such as the production of a biopharmaceutical product or the cultivation of a cell line. This residual DNA can potentially contaminate the final product and affect its safety and efficacy. In the context of biopharmaceutical production, regulatory agencies such as the FDA and EMA have set limits on the amount of residual host cell DNA that is acceptable in a final product. These limits vary depending on the type of product and the route of administration, and residue host cell DNA quantitative kits are designed to ensure that the final product is safe for human use.
Takara Bio PrimeScript™ RT Reagent Kit (Perfect Real Time)
PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which features excellent extension. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. This kit's protocol is simple and suitable for high throughput analysis. It can be used in combination with real-time PCR reagents such as TB Green Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A/B), TB Green Fast qPCR Mix (Cat. #RR430A/B), TB Green Premix Ex Taq (Tli RNaseH Pluse) (Cat. #RR420A/B), or Probe qPCR Mix (Cat. #RR391A/B) for two step real-time RT-PCR. The optimized protocol for assay can be selected in each assay condition using either TB Green or a probe.
New England Biolabs, Inc. Luna® Universal One-Step RT-qPCR Kit – 500 reactions
In the Luna One-Step RT-qPCR Kit, Hot Start Taq DNA Polymerase is combined with a novel WarmStart-activated reverse transcriptase, allowing dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps to prevent undesirable non-specific priming and extension prior to thermocycling, providing added security for setting up reactions at room temperature. The engineered WarmStart Luna Reverse Transcriptase also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. For difficult targets/templates, higher RT step temperatures of up to 60°C can be used without compromising Luna performance. Note that to ensure full activation of the WarmStart Luna RT, incubation at temperatures lower than 50°C is not recommended.
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
Biotium PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA) PMAxx™
This PMAxx™-PCR kit is designed for selective detection of viable bacteria from a specific strain using PMAxx™ dye and real-time PCR. This kit provides primers for specific amplification of the Salmonella enterica invA gene. The kit has sufficient reagents for treating of 80 bacterial cultures and performing 200 PCR reactions with PMAxx™. PMAxx™ is a new and improved version of PMA designed by Biotium scientists to be a superior alternative to PMA. While PMA is generally effective at differentiating between live and dead bacteria by qPCR, it does not completely eliminate PCR products from dead cell DNA. This could potentially give false positive results. Biotium’s new dye PMAxx™ is much more effective at eliminating PCR amplification of dead cell DNA, and therefore provides the best discrimination between live and dead bacteria. Salmonella enterica is a gram-negative bacteria that causes the food-borne illness salmonellosis.
Sigma Aldrich Fine Chemicals Biosciences Betaine solution 5 M, PCR Reagent | 107-43-7 | MFCD00012123 | 5VL
Betaine solution 5 M, PCR Reagent | Mol Wt: 117.15 | 107-43-7 | MFCD00012123 | 5VL
Sciencell Research Laboratories Absolute Human Telomere Length Quantification qPCR Assay Kit 100 reactions
ScienCell's Absolute Human Telomere Length Quantification qPCR Assay Kit (AHTLQ) is designed to directly measure the average telomere length of a human cell population.
INTACT GENOMICS INC igScript™ Probe-Based qPCR master mix 2500 reactions
IgScript™ Probe-Based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye in a standard buffer. It provides improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ Probe-Based qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase Enhanced efficiency, specificity, and sensitivity. Compatible with all real-time PCR instruments for Gene expression validation, multiplexing, mutation detection, pathogen detection and GMO characterization.
INTACT GENOMICS INC ig SYBR Green qPCR 2X Master Mix 500 Reactions
Intact Genomics SYBR Green qPCR 2x master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA by qPCR. The Ig SYBR Green qPCR 2x master mix with integrated chemically modified hot start Taq DNA polymerase, SYBR Green I fluorescent dye, ROX dye*, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. This features a high-quality hot start Taq DNA Polymerase for higher fidelity and better amplification.Applications Gene expression validation Absolute quantification Mutation detection Pathogen detection GMO characterization Genetic profilingBenefits Enhanced efficiency, specificity, and sensitivity Compatible with all real-time PCR instruments Superior gene expression results under various cycling conditions Robust and active for cDNA synthesis at temperatures up to 55 °C.
NEW ENGLAND BIOGROUP LUNA 2nd General Reusable Slide, Counts 1 sample at a time, Contains LUNA reusable slide and one glass coverslip, For brightfield and fluorescent cell counts.
LUNA 2nd General Reusable Slide. Counts 1 sample at a time. Contains LUNA reusable slide and one glass coverslip. Can be used for brightfield and fluorescent cell counts. One year warranty
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG - 500 rxns
The Luna Probe One-Step RT-qPCR 4X Mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.
New England Biolabs, Inc. Luna Probe One-Step RT-qPCR 4X Mix with UDG - 1000 rxns
The Luna Probe One-Step RT-qPCR 4X Mix with UDG is designed for real-time detection of target RNA sequences using hydrolysis probes. In a single tube, RNA is first converted to cDNA by a reverse transcriptase, then a DNA-dependent DNA polymerase amplifies the cDNA, enabling quantitation via real time or quantitative PCR (qPCR). Probe-based qPCR/RT-qPCR monitors an increase in fluorescence upon 5' to 3' exonuclease cleavage of a quenched, target-specific probe to measure DNA amplification at each cycle of a PCR. At a point where the fluorescence signal is confidently detected over the background fluorescence, a quantification cycle or Cq value can be determined. Cq values can be used to evaluate relative target abundance between two or more samples.
As One International Inc ROX internal reference dye, 3 x 0.2 ml
A passive reference dye to compensate for non-PCR related variations in the fluorescence. The fluorescence from the passive reference dye does not change during the course of the PCR reaction but provides a stable baseline to which samples are normalized. The excitation and emission of the reference dye are 584 nm and 612 nm, respectively.
Boca Scientific Inc e-Myco PCR Mycoplasma Detection Kit, 48 Tubes
The e-Myco Mycoplasma PCR Detection Kit consists of a set of primers designed to detect the presence of mycoplasma and determine the species. This kit can detect five common cell culture-infecting types of mycoplasma. The species of mycoplasma can be determined by sequencing the amplified products. 49 species can be detected. Each detection tube contains all components required for PCR in a dried and stable pellet format, including i-StarTaq DNA Polymerase, dNTPs, 10x Buffer, Mycoplasma primers, 8-methoxypsoralen, and an internal control for Mycoplasma partial gene amplifications. The reaction can begin by simply adding the template DNA or samples to be tested. PCR with this primer set is based on conserved 16S rRNA and delivers detection results in only a few hours. This is far more efficient than the conventional detection methods that involve culturing samples on selective media and require at least 1 week to obtain results.